The Pediatric Institute for Heart Regeneration and Therapeutics (I-HRT) is a research institute within the Department of Pediatrics, University of Pittsburgh School of Medicine, directed by Bernhard Kühn, MD. This institute seeks to improve health by repairing hearts through guided regeneration. The institute strives to develop a scientific basis for heart regeneration through world-class research and training. The institute incorporates basic, translational, and clinical research. I-HRT’s goal is to translate the strategy of stimulating heart muscle cell regeneration into a therapy.

Affiliated Faculty:

Bernhard Kühn, MD

Mousumi Moulik, MD

Anita Saraf, MD

Essential Publications

Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division. Jessie W. Yester, Honghai Liu, Frank Gyngard, Niyatie Ammanamanchi, Katherine C. Little, Dawn Thomas, Mara L.G. Sullivan, Sean Lal, Matthew L. Steinhauser, and Bernhard Kühn. Nature Protocols. Feb 24, 2021.

Cardiac Regeneration: Methods and Protocols (ed. Kühn B, Poss K).

Lamin B2 levels regulate polyploidization of cardiomyocyte nuclei and myocardial regeneration.  Han L, Choudhury S, Mich-Basso JD, Ammanamanchi N, Ganapathy G, Suresh S, Khaladkar M, Singh J, Maehr R, Zuppo DA, Kim J, Eberwine JH, Wyman SK, Wu Y & Kühn B. Dev. Cell 2020; 6;53(1):42-59. PMID: 32109383, PMCID: PMC7346764.

Control of Cytokinesis by β-adrenergic Receptors Indicates an Approach for Regulating Cardiomyocyte Endowment. Liu H, Zhang CH, Ammanamanchi N, Suresh S, Lewarchik C, Rao K, Uys GM, Han L, Abrial M, Yimlamai D, Ganapathy B, Guillermier C, Chen N, Khaladkar M, Spaethling J, Eberwine JH, Kim J, Walsh S, Choudhury S, Little K, Francis K, Sharma M, Viegas M, Bais A, Kostka D, Ding J, Bar-Joseph Z, Wu Y, Yechoor V, Moulik M, Johnson J, Weinberg J, Reyes-Múgica M, Steinhauser ML, Kühn B. Science Translational Medicine. 2019 Oct 9.


Pediatric Institute for Heart Regeneration and Therapeutics
John G. Rangos Sr. Research Center, 8th Floor
UPMC Children’s Hospital of Pittsburgh
4401 Penn Avenue
Pittsburgh, PA 15224

Administrative Manager: Maureen Fortunato
phone: 412-692-6256
fax: 412-692-6184


The 12th Annual Heart Institute Research Retreat with featured keynote speaker Shelley Miyamoto, MD on March 29, 2023. 

Agenda and Times