Metabolic Core

The Metabolic Core supports investigators involved in the study of energy metabolism, metabolic pathways, and cellular metabolic function in cells from biological fluids, cell cultures and tissues.

Staff-conducted Services 

  • LC/MS/MS (tandem mass spectrometry)
  • Seahorse extracellular flux analyzer
  • Amino Acid Analyzer
  • HPLC with UV(PDA)/fluorescence/electrochemical detectors

Self-conducted Services

  • Spectrofluorometer – fluorescence measurements and time-dependent (kinetic) assays 
  • Spectrophotometer – absorbance measurements and time-dependent (kinetic) assays

Project Consultation

Technical consultation will be required prior to the initial (first) sample submission.

Pricing

A price list for services is available using the following file link: Price List.

Service Request Form

A service request form is available using the following file link: Service Request Form.  Please save a copy of the completed PDF to your local/network drive, then email the completed Service Request Form to Clint Van't Land (clv19@pitt.edu)

Equipment

LC/MS/MS (tandem mass spectrometry)
AB Sciex® API 4000 triple quadrupole mass spectrometer for small molecule analysis

  • Metabolic profile (concentration) of acylcarnitines, acyl-coenzyme As and other metabolism pathways
  • Inquire about a new assay for your pathway metabolites

Seahorse Extracellular Flux Assays

Agilent® SeahorseTM XFe96 Analyzer
Measurement of mitochondrial function and/or glycolytic activity via:

  • oxygen consumption rate (OCR)
  • extracellular acidification rate (ECAR)

7 kits available – Mito Stress, Glycolysis Stress, Glycolytic Rate, ATP Rate, Cell Energy Phenotype, Mito Fuel Flex, FAO Substrate
www.agilent.com/en/products/cell-analysis

Amino Acid Analyzer
Perkin Elmer® HPLC coupled with a Pickering® post-column reactor (PCX)

  • Assay of 42 amino acids from biological fluids, i.e. plasma (serum) or urine
  • Evaluating a method for cell media

Reverse-Phase HPLC system
Waters® AllianceTM Separations Module with 3 detectors – UV (PDA), fluorescence or electrochemical

  • Analysis of drugs or metabolites
  • Flux studies (substrate vs product)
  • Native protein tryptic mapping (protein-ligand, protein-protein interactions)