New Metabolic Core Facility 

The John G. Rangos Sr. Research Center at UPMC Children's Hospital of Pittsburgh will add a Metabolic Core facility in January of 2019. The new Core supports investigators involved in the study of energy metabolism, metabolic pathways, and cellular metabolic function in cells from biological fluids, cell cultures and tissues.

Staff-conducted services will include:

  • LC/MS/MS (tandem mass spectrometry)
    • Metabolic profile (concentration) of acylcarnitines, acyl-coenzyme As and other metabolism pathways
  • Seahorse extracellular flux analyzer
    • Measurement of mitochondrial function and/or glycolytic activity via:
      • oxygen consumption rate (OCR)
      • extracellular acidification rate (ECAR)
  • Amino Acid Analyzer
    • Assay of 42 amino acids from biological fluids, i.e. plasma (serum) or urine
  • HPLC with UV(PDA)/fluorescence/electrochemical detectors
    • Analysis of drugs or metabolites
    • Flux studies (substrate vs product)
    • Native protein tryptic mapping (protein-ligand, protein-protein interactions)

Self-conducted services will include: 

  • Spectrofluorometer / Spectrophotometer
    • Fluorescence/Absorbance measurements and conducting kinetic studies

Project Consultation:

  • Technical consultation will be required prior to the initial (first) sample submission.

For more information about the Metabolic Core contact the core's director Clint Van’t Land, PhD by phone at 412-692-7652 or by email at clv19@pitt.edu / clinton.vantland@chp.edu or contact staff member Kaitlyn Bloom, PhD by phone at 412-692-9133 or by email at knb52@pitt.edu / kaitlyn.bloom@chp.edu.

More details on the Metabolic Core can be found here.