Anuradha Karunanidhi, BPharm, MS
- Senior Lab Manager Research V
Karunanidhi is a Senior Lab Manager and Research V staff with a background in Pharmaceutical Sciences and Chemical Engineering with 15 years of research experience. She has been part of the Vockley lab for past 10 years. She plays a major role in the ongoing projects involving mitochondrial fatty acid oxidation disorders including ACAD9, CPT II, MCAD, VLCAD, LCAHD, and TFP deficiencies by using her expertise and techniques in designing, developing, screening and optimizing in vitro patients cell culture models to evaluate efficacy of pharmaceutical compounds to remedy the underlying biochemical abnormalities using an array of biomarkers including the effect on production of Reactive Oxygen Species (ROS), immune-imaging on cells and tissues using confocal microscopy, and ATP production levels by high-throughput 96 well plate luminescence assay. Additionally, she has independently trained, identified and custom developed the assays needed for studying key cellular functions, glycolysis, mitochondrial respiration, and the bioenergetic profiling of live patient cells in real time using state-of-art Agilent Seahorse Bioanalyzer instrument. She has trained postdocs, visiting scholars, medical fellows, graduate, and undergraduate students. Her experimental results, and those from other lab members she has taught, have been integrated into multiple published manuscripts and two funded NIH R01 grants. She organizes shared activities among the research group members and has experience in assigned administrative roles including personnel performance evaluation, grant budgetary plans, and equipment purchase suggestions.
Education & Training
- BPharm, The Tamil Nadu Dr. M.G.R. Medical University, India, 1998
- MS, Research in Biochemical Engineering & Biotechnolog, Indian Institute of Technology Madras, India, 2001
Steven F. Dobrowolskia, Ahmad Alodaibb, Anuradha Karunanidhi, Shrabini Basu, Meghan Holeckob, Uta Lichter-Koneckib, Kirk L. Pappand, Jerry Vockley. (2020) Mitochondrial energetics is impaired in very long-chain acyl-CoA dehydrogenase deficiency and can be rescued by treatment with mitochondria-targeted electron scavengers. Molecular Genetics and Metabolism 129 (2020):272-277.
Lina Ghaloul-Gonzalez, Al-Walid Mohsen, Anuradha Karunanidhi, Bianca Seminotti, Hey Chong, Suneeta Madan-Khetarpal, Jessica Sebastian, Catherine Walsh Vockley, Miguel Reyes-Múgica, MarkT.Vander Lugt & Jerry Vockley.(2019). Reticular Dysgenesis and Mitochondriopathy Induced by Adenylate Kinase 2 Defciency with Atypical Presentation.NatureScientific Reports 9:15739
Mateus Grings, Bianca Seminotti, Anuradha Karunanidhi, Lina Ghaloul-Gonzalez, Al-Walid Mohsen, Peter Wipf, Johan Palmfeldt, Jerry Vockley & Guilhian Leipnitz. (2019). ETHE1 and MOCS1 deficiencies: Disruption of mitochondrial bioenergetics, dynamics, redox homeostasis and endoplasmic reticulum-mitochondria crosstalk in patient fibroblasts..NatureScientific Reports9 :12651
Bianca Seminotti, Guilhian Leipnitz, Anuradha Karunanidhi, Catherine Kochersperger, Vera Y. Roginskaya, Shrabani Basu, Yudong Wang, Peter Wipf, Bennett Van Houten, Al-Walid Mohsen and Jerry Vockley. Mitochondrial energetics is impaired in very long-chain acyl-CoA dehydrogenase deficiency and can be rescued by treatment with mitochondria-targeted electron scavengers. Human Molecular Genetics, 2019, Vol. 28 (6):928–941
Leipnitz, G., Mohsen, A.-W.,Karunanidhi, A., Seminotti, B., Roginskaya V., Markantone, D., Grings, M., Mihalik, S., Wipf, P., Van Houten, B., Vockley, J.* (2018). Evaluation of mitochondrial bioenergetics, dynamics, endoplasmic reticulum-mitochondria crosstalk, and reactive oxygen species in fibroblasts from patients with complex I deficiency. NatureScientific Reports8(1):1165
Kaitlyn Bloom; Al-Walid Mohsen; Anuradha Karunanidhi; Dina El Demellawy; Miguel Reyes-Múgica; Yudong Wang; Lina Ghaloul-Gonzalez; Chikara Otsubo; Kimi Tobita; Radhika Muzumdar; Zhenwei Gong; Emir Tas; Jie Chen; Michael Bennett; Charles Hoppel; Jerry Vockley. “ACAD10 genetic defect linked to insulin resistance and obesity". Journal of Inherited Metabolic Disease. 2016
Manuel Schiff, Birgit Haberberger, Chuanwu Xia, Al-Walid Mohsen, Eric S Goetzman, Yudong Wang, Radha Uppala, Yuxun Zhang, Anuradha Karunanidhi, Dolly Prabhu, Hana Alharbi, Edward V Prochownik, Tobias Haack, Johannes Häberle, Arnold Munnich, Agnes Rötig, Robert W Taylor, Robert D Nicholls, Jung-Ja Kim, Holger Prokisch, Jerry Vockley. “Complex I assembly function and fatty acid oxidation enzyme activity of ACAD9 both contribute to disease severity in ACAD9 deficiency”.Hum. Mol. Genet. 2015Jun 26; 24(11):3238-47.
Eric S Goetzman, John F Alcorn, Sivakama S Bharathi, Radha Uppala, Kevin J McHugh, Beata Kosmider, Rimei Chen, Yi Y Zuo, Megan E Beck, Richard W McKinney, Helen Skilling, Kristen R Suhrie, Anuradha Karunanidhi, Renita Yeasted, Chikara Otsubo, Bryon Ellis, Yulia Y Tyurina, Valerian E Kagan, Rama K Mallampalli, Jerry Vockley. (2014). “Long-chain acyl-CoA dehydrogenase deficiency as a cause of pulmonary surfactant dysfunction”.J. Biol. Chem. 289(15):10668-79.
Manuel Schiff, Al-Walid Mohsen, Anuradha Karunanidhi, Elizabeth McCracken, Renita Yeasted, Jerry Vockley. “Molecular and cellular pathology of very-long-chain acyl-CoA dehydrogenase deficiency”Original Research Article. Molecular Genetics and Metabolism, Volume 109, Issue 1, May 2013, Pages 21-27
Young CS, Bradica G, Hart CE, Karunanidhi A, Street RM, Schutte L, Hollinger JO. “Preclinical Toxicology Studies of Recombinant Human Platelet-Derived Growth Factor-BB Either Alone or in Combination with Beta-Tricalcium Phosphate and Type I Collagen”. J Tissue Eng. Jan 10;2010