Bernhard Kühn, MD

  • Professor of Pediatrics and Director, Pediatric Institute for Heart Regeneration and Therapeutics (I-HRT)
  • Associate Director, Richard King Mellon Foundation Institute for Pediatric Research

Understanding the unique workings of heart muscle cells - cardiomyocytes - is the passion of physician-scientist Bernhard Kühn. He and his team of researchers are focused on discovering ways to make cardiomyocytes replicate and proliferate, so as to enable the heart to heal itself in cases of heart failure or congenital defects.

A pioneer in this area of study, Kühn is the recipient of numerous grants and awards, including the American College of Cardiology's prestigious Young Investigator Award, the Basil O'Connor Award from the March of Dimes Birth Defects Foundation, and Scientist Development Grant from the American Heart Association, to name a few.

Professional and Scientific Society Memberships

  • American Academy of Pediatrics, 1999-2008
  • American College of Cardiology, 2002-Present
  • American Heart Association, 2004-Present
  • American Association for the Advancement of Science, 2007-Present
  • American Society for Clinical Investigation, 2016-Present
  • American Pediatrics Society, 2022-Present

Education & Training

  • MD, Freie Universität Berlin, 1999
  • Resident Physician, Yale-New Haven Hospital, 1999-2002
  • Clinical Fellow, Boston Children's Hospital/Harvard Medical School, 2002-2007
  • Post-doctoral Research Fellow, Boston Children's Hospital/Harvard Medical School, 2004-2005

Selected Publications

Han L, Mich-Basso JD, Li Y, Ammanamanchi N, Xu J, Bargaje A, Liu H, Wu L, Jeong JH, Franks J, Stolz DB,Wu YL, Rajasundaram D, Liu Y & Kühn B. Changes in nuclear pore numbers control nuclear import and stress response of mouse hearts. Dev. Cell 2022; 57(20): 2397-2411. PMID: 36283391.

Editorial: Voges L & Krupickova S Intern. J. of Cardiol. 2021; 342: 39-40

El Khoudary SR, Fabio A, Yester JW, Steinhauser ML, Christopher AB, Gyngard F, Adams PA, Morell VO, Viegas M, DaSilva J, Da Fonseca Da Silva L, Castro-Medina M, McCormick A, Reyes-Múgica M, BarlasM , Liu H, Thomas D, Ammanamanchi A, Sada R, Cuda M, Hartigan E, Groscost DK & Kühn B. Design and rationale of a clinical trial to increase cardiomyocyte division in infants with tetralogy of Fallot. Intern. J. of Cardiol. 2021; 339: 36-42

Yester JW, Liu H, Gyngard F, Ammanamanchi N, Little KC, Thomas D, Sullivan MLG, Lal S, Steinhauser ML & Kühn B. Use of stable isotope-tagged thymidine and multi-isotope imaging mass spectrometry (MIMS) for quantification of human cardiomyocyte division. Nature Protocols 2021; 16(4): 1995-2022

Han L, Choudhury S, Mich-Basso JD, Ammanamanchi N, Ganapathy G, Suresh S, Khaladkar M, Singh J, Maehr R, Zuppo DA, Kim J, Eberwine JH, Wyman SK, Wu Y & Kühn B. Lamin B2 levels regulate polyploidization of cardiomyocyte nuclei and myocardial regeneration. Dev. Cell 2020; 53(1): 42-59. PMID: 32109383, PMCID: PMC7346764

Liu H, Zhang C-H, Ammanamanchi N, Suresh S, Lewarchik C, Rao K, Uys GM, Han L, Abrial M, Yimlamai D, Ganapathy B, Guillermier C, Chen N, Khaladkar M, Spaethling J, Eberwine JH, Kim J, Walsh S, Choudhury S, Little K, Francis K, Sharma M, Viegas M, Bais A, Kostka D, Ding J, Bar-Joseph Z, Wu Y, Yechoor V, Moulik M, Johnson J, Weinberg J, Reyes-Múgica M, Steinhauser ML & Kühn B. Control of cytokinesis by β-adrenergic receptors indicates an approach for regulating cardiomyocyte endowment. Sci. Transl. Med. 2019; 11(513): eaaw6419. PMID: 31597755, PMCID: PMC8132604

Ganapathy B, Polizzotti BD, Bennett D, Nandhagopal N, Kühn B. Neuregulin-1 administration protocols sufficient for stimulating cardiac regeneration in young mice do not induce somatic, organ, or neoplastic growth.PLoS One 2016; 11(5): e0155456, PMID: 27175488, PMCID: PMC4866786

Polizzotti BD, Ganapathy B, Haubner BJ, Penninger JM, Kühn B. A cryoinjury model in neonatal mice for cardiac translational and regeneration research. Nature Protocols 2016; 11: 542–552, PMID: 26890681.

Polizzotti BD, Ganapathy B, Walsh S, Choudhury S, Ammanamanchi N, Bennet D, dos Remedios C, Haubner BJ, Penninger JM, Kühn B. Stimulation of cardiomyocyte regeneration in neonatal mice and in human myocardium with neuregulin reveals a therapeutic window. Sci. Transl. Med. 2015;7(281):281ra45. PMCID: PMC5360874

Full Publication List via NIH PubMed

Research Grants

Mechanistic clinical trial of ß-blocker administration for reactivating cardiomyocyte division in Tetralogy of Fallot, (PI, effort 22%), 2021-2026

Quantification of the decline of heart muscle cell proliferation and its reversal in pediatric patients, (PI, effort 35%), 2020-2024

Lamin B2 regulates nuclear remodeling in cardiomyocyte terminal differentiation, (PI, effort 38%), 2020-2024