Available Services

Staff-assisted Services

  • Seahorse XFe96 Analyzer
    • Measurement of mitochondrial function and/or glycolytic activity
      • oxygen consumption rate (OCR)
      • extracellular acidification rate (ECAR)

Staff-conducted Services 

  • LC/MS/MS - Metabolic profiles of pathway intermediates
    • FIA-MS/MS (Flow injection analysis):  Run Times ≤ 4 minutes per injection
      • Acylcarnitines (54), acylglycines (3), and amino acids (9)
    • LCA-MS/MS (LC column analysis):  Run Times > 4 minutes per injection using a HPLC column
      • Qualified assay for S-adenosylmethionine/S-adenosylhomocysteine
  • HPLC - Metabolite analysis using a suitable detector (UV/VIS, fluorescence, or ECD)
    • Neurotransmitters / Monoamine metabolites by HPLC-ECD
      • Qualified assay for Serotonin (5-HT) and 5-HIAA (5-hydroxyindoleacetic acid) 

Spectrometer Services (Discontinued)

The  Metabolic Core will no longer charge fees for use of the Spectrofluorometer and Spectrophotometer. However, the Vockley lab will continue to make the instruments available for use by other labs.